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anti 41bbl  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti 41bbl
    Anti 41bbl, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+41bbl/4-1BBL%2FCD137L%2FTNFSF9+Rabbit+mAb/pmc11784344__jitc___13___1___s001-42-18-22
    Average 93 stars, based on 2 article reviews
    anti 41bbl - by Bioz Stars, 2026-09
    93/100 stars

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    Western Blot:

    Article Title: Plasma membrane-coated nanoparticles and membrane vesicles to orchestrate multimodal antitumor immunity
    Article Snippet: Protein concentration was quantified by the BCA Protein Assay Kit (Takara Bio). .. 9 The following antibodies were used for the immunoblot analysis: anti-CD80 (clone E3Q9V, 10 #15416, Cell Signaling Technology), anti-41BBL (clone E8W3P, #90204, Cell Signaling 11 Technology), anti-SDHA (#5839, Cell Signaling Technology), anti-β-actin (clone C4, sc-47778, 12 Santa Cruz Biotechnology), anti-GAPDH (clone 14C10, #2118, Cell Signaling Technology), 13 anti-CD8α (clone D8A8Y, #85336, Cell Signaling Technology), anti-CD45 (clone D9M8I, 14 #13917, Cell Signaling Technology), HRP-conjugated goat anti-mouse IgG (H + L) (W4021, 15 Promega), and anti-rabbit IgG (W4011, Promega). .. Chemiluminescence was detected with 16 ImmunoStar LD (Fujifilm Wako Pure Chemical, Osaka, Japan) using FUSION-SOLO.7S.EDGE 17 (Vilber Bio Imaging, Paris, France).



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    Fig. 3. Taurine might influence tumor immune response through the Nfe2l1-ROS-PD-1 axis in immune-competent mice. (A-B) RNA sequencing revealed GO analyses and 100 DEGs in subcutaneous lung tumors of C57 mice induced by different doses of taurine (n = 3). (C) Seven representative DEGs were finally identified among the taurine low, taurine high and model group. (D) Different taurine doses significantly affected the expression of Nfe2l1, <t>41BBL,</t> and PD-1 proteins in tumors of C57 mice, as determined by western blot assay (n = 4). (E) For in vivo experiments, LLC (wt) or LLC (Nfe2l1 overexpression) cells were injected by subcutaneously into wild type C57 mice (5 105 cells per mouse, n = 6). (F) Overexpressed Nfe2l1 gene did not significantly affect mouse body weight. (G) Overexpressed Nfe2l1 gene could significantly inhibit LLC-xenografted tumor growth in C57 mice. (H) Representative images of tumors revealed significant anti-tumor effects of overexpressed Nfe2l1 gene in C57 mice. (I) Overexpressed Nfe2l1 gene could cause a decrease in tumor weight. (J) Overexpressed Nfe2l1 gene significantly affected the expression of Nfe2l1, 41BBL, and PD-1 proteins in tumors of C57 mice (n = 4). Statistical significance was calculated by means of ANOVA analysis, *p < 0.05, **p < 0.01, and ***p < 0.001.
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    Fig. 3. Taurine might influence tumor immune response through the Nfe2l1-ROS-PD-1 axis in immune-competent mice. (A-B) RNA sequencing revealed GO analyses and 100 DEGs in subcutaneous lung tumors of C57 mice induced by different doses of taurine (n = 3). (C) Seven representative DEGs were finally identified among the taurine low, taurine high and model group. (D) Different taurine doses significantly affected the expression of Nfe2l1, <t>41BBL,</t> and PD-1 proteins in tumors of C57 mice, as determined by western blot assay (n = 4). (E) For in vivo experiments, LLC (wt) or LLC (Nfe2l1 overexpression) cells were injected by subcutaneously into wild type C57 mice (5 105 cells per mouse, n = 6). (F) Overexpressed Nfe2l1 gene did not significantly affect mouse body weight. (G) Overexpressed Nfe2l1 gene could significantly inhibit LLC-xenografted tumor growth in C57 mice. (H) Representative images of tumors revealed significant anti-tumor effects of overexpressed Nfe2l1 gene in C57 mice. (I) Overexpressed Nfe2l1 gene could cause a decrease in tumor weight. (J) Overexpressed Nfe2l1 gene significantly affected the expression of Nfe2l1, 41BBL, and PD-1 proteins in tumors of C57 mice (n = 4). Statistical significance was calculated by means of ANOVA analysis, *p < 0.05, **p < 0.01, and ***p < 0.001.
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    Fig. 3. Taurine might influence tumor immune response through the Nfe2l1-ROS-PD-1 axis in immune-competent mice. (A-B) RNA sequencing revealed GO analyses and 100 DEGs in subcutaneous lung tumors of C57 mice induced by different doses of taurine (n = 3). (C) Seven representative DEGs were finally identified among the taurine low, taurine high and model group. (D) Different taurine doses significantly affected the expression of Nfe2l1, <t>41BBL,</t> and PD-1 proteins in tumors of C57 mice, as determined by western blot assay (n = 4). (E) For in vivo experiments, LLC (wt) or LLC (Nfe2l1 overexpression) cells were injected by subcutaneously into wild type C57 mice (5 105 cells per mouse, n = 6). (F) Overexpressed Nfe2l1 gene did not significantly affect mouse body weight. (G) Overexpressed Nfe2l1 gene could significantly inhibit LLC-xenografted tumor growth in C57 mice. (H) Representative images of tumors revealed significant anti-tumor effects of overexpressed Nfe2l1 gene in C57 mice. (I) Overexpressed Nfe2l1 gene could cause a decrease in tumor weight. (J) Overexpressed Nfe2l1 gene significantly affected the expression of Nfe2l1, 41BBL, and PD-1 proteins in tumors of C57 mice (n = 4). Statistical significance was calculated by means of ANOVA analysis, *p < 0.05, **p < 0.01, and ***p < 0.001.
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    Fig. 3. Taurine might influence tumor immune response through the Nfe2l1-ROS-PD-1 axis in immune-competent mice. (A-B) RNA sequencing revealed GO analyses and 100 DEGs in subcutaneous lung tumors of C57 mice induced by different doses of taurine (n = 3). (C) Seven representative DEGs were finally identified among the taurine low, taurine high and model group. (D) Different taurine doses significantly affected the expression of Nfe2l1, 41BBL, and PD-1 proteins in tumors of C57 mice, as determined by western blot assay (n = 4). (E) For in vivo experiments, LLC (wt) or LLC (Nfe2l1 overexpression) cells were injected by subcutaneously into wild type C57 mice (5 105 cells per mouse, n = 6). (F) Overexpressed Nfe2l1 gene did not significantly affect mouse body weight. (G) Overexpressed Nfe2l1 gene could significantly inhibit LLC-xenografted tumor growth in C57 mice. (H) Representative images of tumors revealed significant anti-tumor effects of overexpressed Nfe2l1 gene in C57 mice. (I) Overexpressed Nfe2l1 gene could cause a decrease in tumor weight. (J) Overexpressed Nfe2l1 gene significantly affected the expression of Nfe2l1, 41BBL, and PD-1 proteins in tumors of C57 mice (n = 4). Statistical significance was calculated by means of ANOVA analysis, *p < 0.05, **p < 0.01, and ***p < 0.001.

    Journal: Journal of advanced research

    Article Title: Serum taurine affects lung cancer progression by regulating tumor immune escape mediated by the immune microenvironment.

    doi: 10.1016/j.jare.2024.09.005

    Figure Lengend Snippet: Fig. 3. Taurine might influence tumor immune response through the Nfe2l1-ROS-PD-1 axis in immune-competent mice. (A-B) RNA sequencing revealed GO analyses and 100 DEGs in subcutaneous lung tumors of C57 mice induced by different doses of taurine (n = 3). (C) Seven representative DEGs were finally identified among the taurine low, taurine high and model group. (D) Different taurine doses significantly affected the expression of Nfe2l1, 41BBL, and PD-1 proteins in tumors of C57 mice, as determined by western blot assay (n = 4). (E) For in vivo experiments, LLC (wt) or LLC (Nfe2l1 overexpression) cells were injected by subcutaneously into wild type C57 mice (5 105 cells per mouse, n = 6). (F) Overexpressed Nfe2l1 gene did not significantly affect mouse body weight. (G) Overexpressed Nfe2l1 gene could significantly inhibit LLC-xenografted tumor growth in C57 mice. (H) Representative images of tumors revealed significant anti-tumor effects of overexpressed Nfe2l1 gene in C57 mice. (I) Overexpressed Nfe2l1 gene could cause a decrease in tumor weight. (J) Overexpressed Nfe2l1 gene significantly affected the expression of Nfe2l1, 41BBL, and PD-1 proteins in tumors of C57 mice (n = 4). Statistical significance was calculated by means of ANOVA analysis, *p < 0.05, **p < 0.01, and ***p < 0.001.

    Article Snippet: The following antibodies were used in this experiment: b-Actin (CST#8457), Nfe2l1 (Nrf1, SANTA: sc-28379), 41BBL (CD137L, SANTA: sc-398933), PD-1 (CST#84651), NF-jB (CST#8242), and p-NF-jB (CST#3033).

    Techniques: RNA Sequencing, Expressing, Western Blot, In Vivo, Over Expression, Injection